Search results for "Glomus mosseae"
showing 6 items of 6 documents
Changes in polypeptide profiles of two pea genotypes inoculated with the arbuscular mycorrhizal fungus Glomus mosseae
1994
The symbiotic interaction between pea roots ('Pisum sativum' L.) and the endomycorrhizal fungus 'Glomus mosseae' should lead to specific gene expression of both symbionts. In order to detect symbiosis-related proteins (endomycorrhizins), we used two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) after phenolic extraction of total soluble proteins. Furthermore, to better characterize the molecular changes after fungal infection, two pea genotypes available in our laboratory were used: cv. Frisson (myc⁺) and an isogenic mycorrhiza-resistant mutant (myc⁻). The latter prevents intraradical fungal development. Several differences were observed in polypeptide patterns of silver-stained …
Plant gene responses to Glomus mosseae in compatible and incompatible genotypes of Medicago truncatula
2006
International audience
Expression of Sorghum bicolor ammonium transporters upon colonization with arbuscular mycorrhizal fungi
2012
Arbuscular mycorrhizal fungi (AMF) are important plant symbionts, trading mineral nutrients beyond the reach of roots, in particular ammonium, in exchange to their host’s photosynthetic products. Sorghum bicolor is one of the world's leading cereal crops, providing food, fibre and fuel across a range of environments and production systems. It has a particular ability to be productive even under strongly adverse conditions, tolerating much more severe drought than most other grain crops. As its genome has recently been sequenced, we have characterized all eight members of the ammonium transporter (AMT) family and studied their expression in different tissues of field-grown plants. While most…
Colonization of adventitious roots ofMedicago truncatulabyPseudomonas fluorescensC7R12 as affected by arbuscular mycorrhiza
2008
Pseudomonas fluorescens C7R12 was previously shown to promote colonization of Medicago truncatula roots by Glomus mosseae BEG12. To gain more insight into the interaction between C7R12 and BEG12, the cell organization of C7R12 was characterized on adventitious roots mycorrhized or not with BEG12 and on extraradical hyphae. Bacterial cell observations were made using the immuno-fluorescence technique and confocal laser scanning microscopy. Five types of cell organization, so-called organization types (OT), were identified: small or large single cells, cells by pair and cells in microcolonies or in strings. The frequencies of each OT on the roots were expressed as the percentage of observatio…
Gènes fongiques liés au calcium impliqués dans la mycorhize à arbuscules
2012
Fluctuations in intracellular (Ca2+) calcium levels generate signaling events and regulate different cellular processes. Whilst the implication of Ca2+ in plant cell responses during arbuscular mycorrhiza (AM) interactions is well documented, nothing is known about the regulation or role of this secondary meesenger in the fungal symbiont. The molecular basis of fungal calcium homeostasis in the AM symbiosis was analyzed by investigating the expression of Ca2+-related fungal genes. In a first study, G. mosseae genes putatively encoding a MAP3k-like protein kinase (Gm2) and a P-type ATPase (Gm152) were investigated. Both Ca2+-related genes were up-regulated by A. sinicum root exudates, sugges…
Arbuscular mycorhizal proteomes: what news at the nearby and distant horizon?
2007
International audience; Proteomics has soon emerged as a powerful tool to point out protein modifications in roots interacting with arbuscular mycorrhiza (AM) fungi. Depending on the developmental mycorrhizal stage and on the available amount of mycorrhizal material, untargeted and/or sub-cellular proteomic approaches were applied to reveal and identify proteins whose accumulation was modified during the AM colonisation of Medicago truncatula roots. For the early stage of symbiosis, the protein patterns obtained from noninoculated roots and roots synchronized for appressorium formation in wild-type (Jemalong J5), penetration-defective (TRV25, dmi3) and autoregulation-defective (TR122, sunn)…